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Received date : 28-01-2024 Revised date : 09-03-2024 Accepted date : 14-03-2024 Published date : 31-03-2024

Mediterr J Pharm Pharm Sci 4 (1): 121-125, 2024

DOI: https://doi.org/10.5281/zenodo.10814722

Original Research


Chiral screening approach of atorvastatin diastereomers by HPLC method

Thinhinane Hamache, Nasser Belboukhari, Khaled Sekkoum, Marwa Ghouizi



Abstract :

The current study seeks to develop and validate a high-performance liquid chromatography method for atorvastatin diastereomer separation and analysis. In particular, we wish to identify the many diastereomers in atorvastatin, which can help us to better understand their pharmacological properties and provide significant information for pharmaceutical applications. Atorvastatin was chromatographed on a Chiralcel® OD-RH column and n-hexan-2-propanol (95:05 v/v) as the mobile phase, with an injection volume of 10 µL. The solution was pumped at a continuous flow rate of 1 mL/min, with a detection wavelength of 260 nm. The investigation found two peaks with retention times of 3.23 and 3.85 min, respectively. The resolution, capacity, and selectivity factors obtained were Rs = 1.2, k′1=3.50, k′2=4.37, and α=1.24.

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Citation :

Hamache et al. (2024) Chiral screening approach of atorvastatin diastereomers by HPLC method. Mediterr J Pharm Pharm Sci. 4 (1): 121-125. [Article number: 150]. https://doi.org/10.5281/zenodo.10814722

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