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Received date : 09-06-2023 Revised date : 20-07-2023 Accepted date : 25-07-2023 Published date : 30-09-2023

Mediterr J Pharm Pharm Sci 3 (3): 9-15, 2023

DOI: https://doi.org/10.5281/zenodo.8190815

Original Research

Effect of in-vitro differentiated bone marrow mesenchymal stem cells in the treatment of peripheral nerve injury in rats

Atmaram T., Saraswathi Perumal, Balaji Karuppaiah, Saravanakumar S., Nirmal Kumar, and Dhastagir S. Sheriff

Abstract :

Peripheral nerves are more prone to damage during trauma. Though nerve grafts are used as an alternative method in treating it, the results are purely ambiguous. One such modern approach to treat peripheral nerve injury is bone marrow differentiated neuronal cells. Our present aim is to study the effect of in-vitro differentiated bone marrow mesenchymal stem cells (BMSCs) in the treatment of peripheral nerve injury in rats. Six weeks old rat weighing 80 gm was used for isolation and culture of BMSCs. The second passage cells were taken for neuronal differentiation. Flow cytometry and immunocytochemistry were performed with Anti goat IgG antibody indirectly conjugated with FITC to express nestin. In vitro differentiated BMSC along with PLGA Scaffold is injected into the site of peripheral nerve injury and the results were studied by ENMG, microdissection, and histopathology. The cells were expressed with Nestin goat polyclonal antibody. At the end of the second week, the rat reveals increased amplitude (8.3 mv) with decreased latency (0.8 ms) of the peripheral nerve. Micro dissection confirms the neuronal continuity of the injured peripheral nerve. Histopathology distinctly exhibit increased myelination and decreased endoneuronal space. In conclusion, neuronal differentiated BMSC, regenerate peripheral nerve injury faster than conventional methods and can be applied as an alternate therapy in peripheral nerve repair.

Tissue engineering is multifaceted, involves the isolation, characterization and differentiation of bone marrow cells. Transplantation of neuronal differentiated bone marrow mesenchymal stem cells (BMSCs) onto the site of injury results in peripheral nerve repair. This method of clinical transplantation of differentiated neuronal cells along with bioscaffolds challenges regenerative medicine for faster and complete nerve repair. Autologous nerve grafts had been the gold standard for the treatment of the peripheral nerve injury that exceeded the critical gap length. Transplantation of differentiated BMSCs into Schwann cells along with a bioengineered scaffold holds promise for nerve regeneration because of the limited availability of donor nerves and donor morbidity [1]. BMSCs are supposed to be the ideal transplantable cell due to its easy accessibility, rapid expansion capacity when cultured, immune-competent and immune-privileged nature [2]. Recent transplantation studies suggest in-vitro differentiation of BMSCs into cells expressing Schwann cell antigen, followed by in-vivo transp-lantation, shown to have a faster regenerative effect on damaged sciatic nerve [3]. This finding suggests that in-vivo transplantation of BMSCs into a central and peripheral nervous system following ex-vivo differentiation makes it potential to fill nerve gap repair. The main purpose of this study is ex-vivo differentiation of BMSCs into neuronal cells and in-vivo transplantation of differentiated neuronal cells with poly lactic co glycolic acid (PLGA) scaffold aids faster regeneration of nerve gaps in trauma, gunshot and neurodegenerative diseases.


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Citation :

Atmaram et al. (2023) Effect of in-vitro differentiated bone marrow mesenchymal stem cells in the treatment of peripheral nerve injury in rats. Mediterr J Pharm Pharm Sci. 3 (3): 9-15. https://doi.org/10.5281/zenodo.8190815.

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