Original Research
Effect of resveratrol on total protein and albumin in type 2 diabetes wound healing in rats
Naim O. Yusuf, Aisha Idris, Jimoh Abdulazeez, Praise J. Folorunsho, Haruna A. Isah and Fatima A. Dawud
Abstract :
Resveratrol, a polyphenol predominantly present in red grapes, has attracted interest due to its possible health advantages. The anti-inflammatory, antioxidant, and vasodilatory properties of this substance indicate that it may have a beneficial effect on wound healing in individuals with diabetes mellitus. This study aims to determine the role of resveratrol on type 2 diabetic wound healing on total protein and albumin levels in rats. 20 male adult Albino Wistar rats were rendered diabetic using a high-fat diet and an alloxan injection (120 mg/kg). The rats were grouped into four: non-diabetic control group (negative control), diabetic control group (positive control), diabetic treatment group 1 (resveratrol: 10 mg/kg) and diabetic treatment group 2 (resveratrol: 20 mg/kg). Excisional wounds were created and monitored for wound closure over a defined treatment period of 14 days. Studies were conducted and expressed using physical and biochemical indices. The data demonstrated wound healing activities via biochemical indices, and histological and macroscopic methods. There was a difference in fasting blood glucose between the diabetic control group with the treatment groups. There was a difference between the diabetic control group compared to the group treated with resveratrol 10 mg/kg and 20 mg/kg in the weight of the rats. There was no significant acceleration in total albumin and globulin levels in the diabetic wounded group treated with resveratrol (10 mg and 20 mg). The results suggest that resveratrol treatment does not affect on total albumin and globulin levels in diabetic rats.
References
1. Liu Y, Liu Y, He W, Mu X, Wu X, Deng J, Nie X (2022) Fibroblasts: immunomodulatory factors in refractory diabetic wound healing. Frontiers in Immunology. 13. 918223. doi: 10.3389/fimmu.2022.918223
2. Dietrich I, Braga GA, de Melo FG, da Costa Silva Silva ACC (2017) The diabetic foot as a proxy for cardiovascular events and mortality review. Current Atherosclerosis Reports. 19 (11): 44. doi: 10.1007/s11883-017-0680-z
3. Habegger KM, Heppner KM, Geary N, Bartness TJ, DiMarchi R, Tschöp MH (2010) The metabolic actions of glucagon revisited. Nature Reviews, Endocrinology. 6 (12): 689-697. doi: 10.1038/nrendo.2010.187
4. Sharp A, Clark J (2011) Diabetes and its effects on wound healing. Nursing Standard. 25 (45): 41-47. doi: 10.7748/ ns2011.07.25.45.41.c8626
5. Spampinato SF, Caruso GI, De Pasquale R, Sortino MA, Merlo S (2020) The treatment of impaired wound healing in diabetes: looking among old drugs. Pharmaceuticals. 13 (4): 60. doi: 10.3390/ph13040060
6. Nicolucci Ac (2011) Fact and figures about diabetes in Italy. Assistenza Infermieristica e Ricerca. 30 (2): 100-106. doi: 10.1702/845.9396
7. Mohandas A, Anisha B, Chennazhi K, Jayakumar R (2015) Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids and Surfaces B, Biointerfaces. 127: 105-113. doi: 10.1016/j.colsurfb.2015.01.024
8. Nathan DM, Buse JB, Davidson MB, Heine RJ, Holman RR, Sherwin R, Zinman B (2006) Management of hyperglycemia in type 2 diabetes: A consensus algorithm for the initiation and adjustment of therapy: a consensus statement from the American Diabetes Association and the European Association for the Study of Diabetes. Diabetes Care. 29 (8): 1963-1972. doi: 10.2337/dc06-9912
9. Crowe T, Brockbank C (2009) Nutrition therapy in the prevention and treatment of pressure ulcers. Wound Practice and Research. 17 (2): 90. 92-99. doi: Nil.
10. Thompson C, Fuhrman MP (2005) Nutrients and wound healing: still searching for the magic bullet. Nutrition in Clinical Practice. 20 (3): 331-347. doi: 10.1177/0115426505020003331
11. Sajid N, Miyan Z, Zaidi SIH, Jaffri SSA, AbdeAli M (2018) Protein requirement and its intake in subjects with diabetic foot ulcers at a tertiary care hospital. Pakistan Journal of Medical Sciences. 34 (4): 886-890. doi: 10.12669/pjms.344.15399
12. Hung L-M, Su M-J, Chen J-K (2004) Resveratrol protects myocardial ischemia–reperfusion injury through both NO-dependent and NO-independent mechanisms. Free Radical Biology and Medicine. 36 (6): 774-781. doi: 10. 1016/j.freeradbiomed.2003.12.016
13. Liu K, Zhou R, Wang B, Mi M-T (2014) Effect of resveratrol on glucose control and insulin sensitivity: a meta-analysis of 11 randomized controlled trials. The American Journal of Clinical Nutrition. 99 (6): 1510-1519. doi: 10.3945/ajcn.113.082024
14. Kavitha KV, Tiwari S, Purandare VB, Khedkar S, Bhosale SS, Unnikrishnan AG (2014) Choice of wound care in diabetic foot ulcer: A practical approach. World Journal of Diabetes. 5 (4): 546-556. doi: 10.4239/wjd.v5.i4.546
15. Boulton AJM, Armstrong DG, Löndahl M, Frykberg RG, Game FL, Edmonds ME, Orgill DP, Kramer K, Gurtner GC, Januszyk M, Vileikyte L (2022) New evidence-based therapies for complex diabetic foot wounds. Arlington (VA): American Diabetes Association; 2022 May. PMID: 35763580.
16. Saikat Sen, Biplab De, Devanna N, Chakraborty R (2013) Hypoglycemic and hypolipidemic effect of Meyna spinosa leaves in high fat diet-alloxan induced type 2 diabetic rats. Bangladesh Journal of Pharmacology. 8 (2): 1-4. doi: 10.3329/bjp.v8i2.14299
17. Alsarayreh AZ, Oran SA, Shakhanbeh JM, Khleifat KM, Al Qaisi YT, Alfarrayeh II, Alkaramseh AM (2022) Efficacy of methanolic extracts of some medicinal plants on wound healing in diabetic rats. Heliyon. 8 (8): e10071. doi: 10.1016/j.heliyon.2022.e10071
18. Yonamine CY, Pinheiro-Machado E, Michalani ML, Freitas HS, Okamoto MM, Corrêa-Giannella ML, Machado UF (2016) Resveratrol improves glycemic control in insulin-treated diabetic rats: participation of the hepatic territory. Nutrition and Metabolism. 13 (1): 44. doi: 10.1186/s12986-016-0103-0
19. Lin YH, Hsu WS, Chung WY, Ko TH, Lin JH (2016) Silver-based wound dressings reduce bacterial burden and promote wound healing. International Wound Journal. 13 (4): 505-511. doi: 10.1111/iwj.12467
20. Park KT, Yun CH, Bae CS, Ahn T (2014) Decreased level of albumin in peripheral blood mononuclear cells of streptozotocin-induced diabetic rats. The Journal of Veterinary Medical Science. 76 (8): 1087-1092. doi: 10.1292/ jvms.13-0631
21. Milton-Laskibar I, Gómez-Zorita S, Aguirre L, Fernández-Quintela A, González M, Portillo MP (2017) Resveratrol -induced effects on body fat differ depending on feeding conditions. Molecules. 22 (12): 2091. doi: 10.3390/ molecules22122091
22. de Ligt M, Bergman M, Fuentes RM, Essers H, Moonen-Kornips E, Havekes B, Schrauwen-Hinderling VB, Schrauwen P (2020) No effect of resveratrol supplementation after 6 months on insulin sensitivity in overweight adults: a randomized trial. The American Journal of Clinical Nutrition. 112 (4): 1029-1038. doi: 10.1093 /ajcn/nqaa125
23. Ramar M, Manikandan B, Raman T, Priyadarsini A, Palanisamy S, Velayudam M, Munusamy A, Prabhu MN, Vaseeharan B (2012) Protective effect of ferulic acid and resveratrol against alloxan-induced diabetes in mice. European Journal of Pharmacology. 690 (1-3): 226-235. doi: 10.1016/j.ejphar.2012.05.019
24. Zhu X, Wu C, Qiu S, Yuan X, Li L (2017) Effects of resveratrol on glucose control and insulin sensitivity in subjects with type 2 diabetes: systematic review and meta-analysis. Nutrition and Metabolism. 14: 60. doi: 10.1186/s12986-017-0217-z
25. Hoseini A, Namazi G, Farrokhian A, Reiner Ž, Aghadavod E, Bahmani F, Asemi Z (2019) The effects of resveratrol on metabolic status in patients with type 2 diabetes mellitus and coronary heart disease. Food and Function. 10 (9): 6042-6051. doi: 10.1039/c9fo01075k
26. Gu W, Geng J, Zhao H, Li X, Song G (2022) Effects of resveratrol on metabolic indicators in patients with type 2 diabetes: a systematic review and meta-analysis. International Journal of Clinical Practice. 2022: 9734738. doi: 10.1155/2022/9734738
27. Soufi FG, Sheervalilou R, Vardiani M, Khalili M, Alipour MR (2012) Chronic resveratrol administration has beneficial effects in experimental model of type 2 diabetic rats. Endocrine Regulations. 46 (2): 83-90. doi: 10.4149 /endo_2012_02_83
28. Timmers S, Konings E, Bilet L, Houtkooper RH, van de Weijer T, Goossens GH, Hoeks J, van der Krieken S, Ryu D, Kersten S, Moonen-Kornips E, Hesselink MKC, Kunz I, Schrauwen-Hinderling VB, Blaak E, Auwerx J, Schrauwen P (2011) Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans. Cell Metabolism. 14 (5): 612-622. doi: 10.1016/j.cmet.2011.10.002
29. Majid A, Sayer SA, Farhood HB (2018) Study of some biochemical parameters for patients with Type II Diabetes Mellitus in Thi-Qar Governorate, Iraq. Journal of Pharmaceutical Sciences and Research. 10 (11): 2938-2941. Corpus ID:212610440.
30. Rabbani N, Thornalley PJ (2021) Protein glycation - biomarkers of metabolic dysfunction and early-stage decline in health in the era of precision medicine. Redox Biology. 42:101920. doi: 10.1016/j.redox.2021.101920
31. Khalaf MA, Zainal IG (2021) Investigation of antioxidant markers in diabetic patients. Archives of Razi Institute. 76 (5): 1453-1460. doi: 10.22092/ari.2021.355755.1717
Citation :
Yusuf et al. (2024) Effect of resveratrol on total protein and albumin in type 2 diabetes wound healing in rats. Mediterr J Pharm Pharm Sci. 4 (1): 126-132. [Article number: 151]. https://doi.org/10.5281/zenodo.10827045