Short Communication
Physicochemical analysis of Terminalia catappa (Almond) seed nuts grown in Benin city, Nigeria
Osaro Iyekowa, Justina E. Ukpebor and Emmanuel Ehigimetor
Introduction
Worldwide,
fruits and sometimes fruit nuts of many plants have been consumed for their
nutritional benefits but, apart from that, they could be of a medical and
pharmaceutical importance use to humans. Terminalia
catappa (T. catappa, almond plant) from Combretaceae family
is a large tree in the Leadwood tree family that grows mainly in tropical
regions of Africa, Asia and Australia [1]. In Southern Nigeria, it is referred to as
“Ebelebo” among the Binis, “Baushe” in Hausa and “Igi-furutu” in Yoruba [2].
The juice obtained from its fresh leaves has
been used in preparation of medicinal lotion which is effective against
scabies, leprosy, stomach ache and headache [3]. The tree provides several other dyes, tannins, timbers, carbohydrates,
fuel plant and many other medicinal purposes [2]. T.
catappa is a well-known herb in Ayurveda traditions in
Indian. The ethno-medical uses of T. catappa
includes modulatory activity of methanol extract of the leaves, wound
healing activity of the chloroform
extract of the bark [4], anticancer potency of the ethanol extract of the
leaves [5] and anti-oxidant, hepato-protective, anti-microbial, analgesic,
anti-inflammatory, anti-diabetic and anti-aging activity [3]. Recent scientific
investigations revealed that the methanol extract of T. catappa leaf extract exhibits the dosage-dependent increase in
inhibitory effect on α-glucosidase enzyme and α-amylase enzyme [6]. T. catappa exhibits anti-tumor activity
with high anti-oxidant levels and the anti-oxidant defense may be due to the
presence of phenolic and flavonoid components [5]. Phytochemical screening of
the tree bark and leaves contains saponins, alkaloids, glycoside, terpenes,
volatile oils, steroid and phenols. While, the seeds are rich in oleic‑linoleic
acid group, steroids, glycosides, phenolics and terpenes [7]. T. catappa has been observed to possess
the anti-oxidant activity in a dose-dependent manner by DPPH assay, nirtic oxide
assay, reducing power assay and H2O2 assay [8]. The plant
also reverses the lipid levels to normal range and this shows anti-tumor and
anti-lipidemic activities [9]. The leaves extract also inhibit the expression
and activities of matrix metallo-proteinase-9 (MMP-9) by the assessment of mRNA
levels in hepato-cellular carcinoma [10]. This research is aimed at determining the proximate composition,
mineral elements and physicochemical parameters of T. catappa seed extract.
Materials
and methods
Materials: All the chemicals used in this work were of
analytical grade and obtained from Merck and Sigma Aldrich, Modderfontein Johannesburg 1645, South Africa.
Sample collection
and treatment: The
fruits of T. catappa were collected
from tree grown in Benin city, Edo State,
Nigeria. The fresh epicarps of the T. catappa
fruit were peeled to expose the seeds. The exposed seeds were then air dried
for seven days and cracked open to collect the nuts. The seed nuts were
air-dried under normal laboratory conditions for four weeks and finally
pulverized. The powder seed nuts were then used for the proximate analysis and
the determination of mineral composition [11].
Proximate
analysis: The proximate composition of the
seed kernel was analyzed following standard methods [11], crude fat by Soxhlet method and moisture by vacuum oven, crude
protein by Kjeldahl method and ash by ignition. Carbohydrate content was calculated by subtracting the values of all
the other proximate analysis from 100 [11].
Mineral element
determination: For the elemental analysis of the powder seeds, one gram
of the sample was digested with concentrated HNO3 and HClO4.
The sample was then filtered and made up to 50 ml with distilled water [11].
The metal concentrations were determined using
an Atomic Absorption Spectrophotometer (Buck Scientific model 210) and a Flame
Spectrophotometer (Sherwood, model 410). The mineral elements investigated were
Iron (Fe), Zinc (Zn), Calcium (Ca), Magnesium (Mg), Potassium (K), Nickel (Ni),
Sodium (Na), Cadmium (Cd), Cupper (Cu), Chromium (Cr) and Lead (Pb).
Physico-chemical
analysis: The physicochemical analysis including acid
value, saponification value, peroxide value and iodine value were determined
according to standard methods [1].
Results
The results of the proximate analysis, mineral
elements and physicochemical analysis of the seed oil extract of T. catappa seed nut are shown in
Tables 1, 2 and 3, respectively. Thus, a wide range of
percentage among the constituents of T.
catappa seed nut was found as shown in Table 1. Thus, carbohydrate,
fat and crude protein were found in high percentage (33.3, 21.9 and 29.6%
respectively) whereas, fibre, ash and moisture contents were found in very low
percentage (08.5, 03.8 and 02.3%, respectively).
Table
1: Results of proximate composition
of T.
catappa seed nut
Parameter |
Value (%) |
Moisture |
02.34
± 0.2 |
Ash |
03.89
± 0.1 |
Fibre
content |
08.50
± 0.1 |
Crude protein |
29.66 ± 0.3 |
Fat |
21.98
± 0.1 |
Carbohydrate |
33.63 ± 0.2 |
In Table 2, different contents of mineral elements of
the extract are given. Potassium, magnesium and zinc contents represent the
highest, whereas, other elements are presented in a very low contents. However,
lead (Pb) content was not detected in this extract.
Table
2: Mineral elements detected in hexane
extract of T.
catappa seed nut
Element |
Content (mg/kg) |
Fe Zn Ca |
06.40
± 0.00 39.00
± 0.42 29.00
± 2.60 |
Mg |
30.00 ± 3.13 |
K |
54.7
± 1.50 |
Ni |
0.00 ±
0.00 |
Na |
2.10
± 0.18 |
Cd |
0.00 ±
0.00 |
Cu |
0.30
± 0.01 |
Cr |
0.18 ± 0.01 |
Pb |
0.00 ± 0.00 |
Table 3 shows acid, saponification, iodine and
peroxide values of the crude oil hexane extract of T. catappa seed nut. A large variation was found among them with saponification
value represents the high value and acid value was the lowest value.
Table
3: Chemical parameters of the crude
oil hexane extract of T. catappa seed nut
Parameter |
|
Acid
value [mg
KOH/g of oil] |
0.102
± 0.01 |
Saponification value [mg
KOH/g of oil] |
151.28 ± 2.63 |
Iodine value [g
I2/100 g of oil] |
47.19
± 1.44 |
Peroxide value [meq/kg
of oil] |
8.93 ± 0.86 |
Discussion
In this study, the moisture content obtained
for the almond seed nuts was to be 02.34%. Moisture content helps to determine
storage time. Low moisture content is important for storage quality and shelf
life of seeds. The moisture content indicates that almond seed can thus be kept
for a long time. Furthermore, most researches have inferred that low moisture
in seeds reduces the microbial activities and decreases unwarranted
fermentation [1]. The lower moisture content recorded may be due to the fact that the
almond seeds were air dried for 28 days. The ash content of a sample is an
indication of the level of minerals present [12]. The values of ash content reported from
different locations in Nigeria appear to fall within a similar range of 02.00% -
05.00%. According to the previous report [13], low ash content of seeds could be credited to
the fact that during maturation, inorganic ions migrate from different parts of
the plant to the region of active growth. This value was also lower than those
obtained for almond seeds from other countries like Ivory Coast (04.60%) but
higher than 03.78% reported in the seeds from Malaysia [14].
The crude fiber content obtained in this work
was 08.50% and higher than 03.11% obtained by Akpabio [12].
The high amount of protein content obtained in
this work suggests that almond nut can be used to supplement other dietary
sources. Therefore, it is recommended that almond nut be added to the meals of
individuals in order to increase the protein content. From
other study, the seed contains carbohydrates, protein, fat, fiber, iron,
ascorbic acid, arachidic acid and β-carotene are in good proportion [15].
Amongst the minerals determined, the
concentration of potassium was the highest (54.7 mg/kg) and Cr had the least quantity
(0.18 mg/kg) while Ni, Cd and Pb were beyond detection limit. Iron had a quantity
of 6.40 mg/kg. On comparison with the work of Mandloi [15],
phosphorus, potassium, niacin, riboflavin and thiamin were also detected in the
seed. Therefore, almond seed could be recommended
as a dietary supplement for people who need essential minerals and iron. The World
health Organization (WHO) recommended daily allowance for zinc is 11 mg.
Therefore, almond nut when properly processed will be a good source for zinc
due to its high value. Potassium had a quantity of 54.7 mg/kg which plays a
role in many body functions including transmission of nerve signals, muscle
contractions, fluid balance and various chemical reactions.
The chemical
parameters of the oil extract shown below in this study indicated an acid value of 0.102 mg KOH/g of oil which
is attributable to its low free fatty acid value of 0.051. This value was low
when compared to 0.787 mg KOH/g of almond nut oil obtained from India [12].
However, Ogbeide and
others [16]
reported a much higher level acid value of 2.94
mg KOH/g. The saponification
value obtained showed a value of 151.278 mg KOH/g which less than the value
326.08 mg KOH/g is obtained [16]. The saponification value obtained in this work could suggest that
the almond seed has a few proportions of low molecular weight fatty acids and
the difference observed could be as a result of method of extraction of the oil
from the seed. Other report, however, indicated that almond nut oil contains
higher proportions of low molecular weight fatty acids [1]. Rancidity begins to occur in oil when the
peroxide value ranges from 20.0 mg/g oil to 40.0 mg/g oil. The peroxide value obtained in this work was 8.93 meq/kg and this low
values of peroxide value are indicative of low levels of oxidative rancidity of
the oils and suggest high levels of antioxidants [1]. It
has been asserted that oils with iodine value less than 100 mg I2/100g
are non-drying oils, and consequently, the lesser the number of unsaturation
the lower the susceptibility of such oil to oxidative rancidity [17]. The iodine value obtained in this work was
47.186 g/100 g which is less than 131.37 g/100 g obtained by [16] and 121.19 g/100 g obtained by [1]. The iodine value obtained was less than 100
g/100 g, thus, almond nut oil cannot be placed in the class of drying oil.
Conclusion: Terminalia catappa seed nuts contain essential minerals required for nutritional and medicinal purposes, and the low moisture content of the oil indicates that products or formulations from the nuts will possess longer shelf life.
References
1. Akpakpan AE, Akpabio UG (2012) Evaluation of proximate composition,
mineral element and anti-nutrient in almond (Terminalia catappa) seeds. Research
Journal of Applied Sciences. 7 (9): 489-493. doi: 10.3923/rjasci.2012.489.493.
2. Aigbokhan EI
(2014) Annotated checklist of vascular plants of Southern Nigeria- a quick reference
guide to the vascular plants of southern Nigeria: a systematic approach. Uniben
Press, Benin City. doi: 10.13140/RG.2.1.1604.0808.
3. Anand AV, Divya N, Kotti PP (2015) An updated review of
Terminalia catappa. Pharmacognosy Reviews. 9 (18): 93-98. doi: 10.4103/0973-7847.162103.
4. Khan AA,
Kumar V, Singh BK, Singh R (2014) Evaluation of wound healing property of Terminalia
catappa on excision wound models in Wistar rats. Drug Research (Stuttg). 64 (5):
225-228. doi: 10.1055/s-0033-1357203.
5. Pandya NB,
Tigari P, Dupadahalli K, Kamurthy H, Nadendla RR (2013) Antitumor and
antioxidant status of Terminalia catappa against Ehrlich ascites carcinoma in Swiss
albino mice. Indian Journal of Pharmacology. 45 (5): 464-469. doi: 10.4103/0253-7613.117754.
6. Divya N,
Vijaya A (2014) Phytochemical investigation and in vitro anti-diabetic activity
of Terminalia catappa leaves. International Journal of Phytopharmacy. 4 (5): 132-134.
doi.org/10.7439/ijpp.v4i5.111.
7. Mininel FJ, Leonardo-Junior
CS, Espanha LG, Resende FA, Varanda EA, Leite COF, Vilegas W, Dos Santos LC (2014)
Characterization and quantification of compounds in the hydroalcoholic extract
of the leaves from Terminalia catappa Linn. (Combretaceae) and their mutagenic
activity. Evidence-Based Complementary and Alternative Medicine. 67-69.
2014: 676902. doi: 10.1155/2014/676902.
8. Kotti PP,
Anand AV (2014) Phytochemical analysis and in vitro antioxidant activity of
Terminalia catappa. World Journal of Pharmaceutical Sciences. 2 (11): 1495-1498.
9. Naitik P,
Prakash T, Kotresha D, Rao NR (2012) Effect of Terminalia catappa on lipid profile
in transplanted fibrosarcoma in rats. Indian Journal of Pharmacology. 44 (3): 390-392. doi: 10.4103/0253-7613.96345.
10. Yeh CB, Yu
YL, Lin, CW, Chiou HL, Hsieh MJ, Yang SF (2014) Terminalia catappa attenuates
urokinase-type plasminogen activator expression through Erk pathways in hepatocellular
carcinoma. BMC Complementary and Alternative Medicine. 14:141.
doi: 10.1186/1472-6882-14-141.
11. Association of Official Analytical Chemists AOAC (2015) Official
methods of analysis of the association of official analytical chemists, 5th
Edn. Association of official analytical chemists, Virginia, USA. ISBN:
0-935584-42-0.
12. Atsu-Barku VY, Nyarko HD, Dordunu P (2012) Studies
on the physicochemical characteristics, microbial load and storage stability of
oil from Indian almond nut (Terminalia catappa). Food Science and Quality
Management. 8: 9-17.
Corpus ID: 73648051.
13. Monnet YT, Gbogouri A, Koffi, PKB, Kouamé LP (2012) Chemical
characterization of seeds and seed oils from mature Terminalia catappa fruits
harvested in Côte d’Ivoire. International Journal of Bioscience. 2: 110-124. Corpus ID: 86147763.
14. Siew NG, Ola L, Muhammad
KS, Hussain
N, Sulaiman R (2015) Physicochemical
properties of Malaysian-grown tropical almond nuts (Terminalia catappa). Journal of Food Science and
Technology. 52
(10): 6623-6630. doi: 10.1007/s13197-015-1737-z.
15. Mandloi S, Mishra
R, Varma R, Varughese B, Tripathi J (2013) A study on phytochemical and
antifungal activity of leaf extracts of Terminalia cattapa. International
Journal of Pharm and BioScience. 4: 1385-1393. Scorpus ID: 13835580.
16. Ogbeide OK,
Eze OF, Akaeze DA, Akhigbe IU, Omoruyi U, Iyekowa O, Owolabi BJ (2021) Physico-chemical
properties, chemical composition, biodiesel production and antibacterial
potential of Terminalia catappa seed oil. ChemSearch Journal. 12 (2): 70-80.
17. Aremu MO,
Ibrahim H, Bamidele TO (2015) Physicochemical characteristics of the oils extracted
from some Nigerian plant foods - A review. Chemical and Process Engineering
Research. 32: 36-52.
Citation :
Iyekowa et al. (2023) Physicochemical analysis of Terminalia catappa (Almond) seed nuts grown in Benin City, Nigeria. Mediterr J Pharm Pharm Sci. 3 (1): 6-10. https://doi.org/10.5281/zenodo.7771322.